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Immobilization of lipase on porous monodisperse chitosan microspheres
Author(s) -
Chen Yang,
Liu Junteng,
Xia Chunjie,
Zhao Chenxi,
Ren Zhongqi,
Zhang Weidong
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1242
Subject(s) - glutaraldehyde , dispersity , lipase , immobilized enzyme , chitosan , chromatography , chemistry , microsphere , enzyme , chemical engineering , polymer chemistry , biochemistry , engineering
Porous monodisperse chitosan microspheres were synthesized for enzyme immobilization. The microspheres were prepared using microchannels and modified with glutaraldehyde. The microspheres had a mean diameter of 495 µm; the polydispersity indices were less than 0.08, and the specific surface area was between 121 and 173 m 2 /g. C andida sp. 1619 lipase was selected as a model lipase. Immobilization conditions such as enzyme loading, glutaraldehyde concentration, and immobilization time were optimized. The temperature, p H , and storage stability of the free and immobilized enzymes were also investigated. The immobilized enzyme had broad‐ranging p H and temperature optima as compared with free enzyme. The storage stability of the immobilized enzyme was higher than that of the free enzyme.