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Preparation and identification of transfer factor specific to S taphylococcus aureus in vitro
Author(s) -
Zhou Jianwei,
Kong Cui,
Wang Xiukui,
Shao Jun,
Feng Lin,
Zhang Zhaocai
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1241
Subject(s) - in vitro , transfer factor , immune system , adjuvant , phagocytosis , spleen , chemistry , staphylococcus aureus , microbiology and biotechnology , immunology , biology , bacteria , biochemistry , genetics
The objective of the study was to explore the methods for preparing transfer factor specific to S taphylococcus aureus ( SA‐STF ) in vitro . Under the optimum conditions, the spleen cells of rabbits were immunized with SA in vitro to prepare SA‐STF , and the immune activities were identified with the phagocytosis and sterilization, skin delayed‐type hypersensitivity, and immune protection tests. The concentration of polypeptide was 2.26 ± 0.27 mg/mL, and ribose was 0.684 ± 0.094 mg/mL. The phagocytosis and sterilization rates of the STF group were 70.9 ± 12.4% and 62.1 ± 12.2%, respectively, and compared with the non‐specific transfer factor ( NTF ) group, there were no significant differences ( P = 0.074 and 0.069, respectively). The skin was inflamed and marked nodules formed at the injection site in the mice of the STF group rather than the NTF and control groups. The survival rate of the STF ‐1 group was significantly higher than the survival rates of the STF ‐2 ( P = 0.024) and NTF groups ( P = 0.016). SA‐STF was prepared and characterized successfully in vitro , and it probably is a biological candidate for therapy or adjuvant therapy for diseases caused by S taphylococcus aureus .