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Development of all‐in‐one multicistronic T et‐ O n lentiviral vectors for inducible co‐expression of two transgenes
Author(s) -
Huang Yide,
Zhen Ruonan,
Jiang Meiqin,
Yang Jie,
Yang Yun,
Huang Zhen,
Lin Yao
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1239
Subject(s) - doxycycline , transactivation , biology , transgene , microbiology and biotechnology , gene , tetracycline , vector (molecular biology) , transcription (linguistics) , expression vector , gene expression , recombinant dna , genetics , antibiotics , linguistics , philosophy
Inducible co‐expression of multiple genes is often needed in research. Here we describe a single‐vector‐based T et‐ O n inducible system for co‐expression of two transgenes. The two transgenes ( D s R ed1 and e GFP as model genes) and reverse tetracycline‐controlled transactivator were separated by internal ribosomal entry sites and 2A sequences, and their transcription was controlled by the same tetracycline responsive element. Two novel vectors with different internal ribosomal entry sites and 2A positions on the vectors were constructed. The D s R ed1 and e GFP in cells transduced with both vectors are undetectable in the absence of doxycycline and can be efficiently induced in the presence of doxycycline in vitro and in vivo . These two vectors can be useful tools when regulated co‐expression of two ecotopic genes is needed.