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Furfural and hydroxymethylfurfural tolerance in Escherichia coli Δ acrR regulatory mutants
Author(s) -
Luhe Annette Lin,
Lim Chan Yuen,
Gerken Henri,
Wu Jinchuan,
Zhao Hua
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1232
Subject(s) - escherichia coli , furfural , mutant , chemistry , microbiology and biotechnology , biology , food science , biochemistry , gene , catalysis
The presence of the highly toxic furfural and hydroxymethylfurfural ( HMF ) in the hydrolysate of lignocellulosic biomass prompted the investigation of the E scherichia coli Δ acrR regulatory mutant for higher tolerance to these compounds, to facilitate the production of biofuels and biochemicals, and further biocatalytic conversions. In comparison with the parental strain, the regulatory mutant with the upregulated efflux pump AcrAB‐TolC produced moderately better growth and higher tolerance to concentrations of furfural and HMF between 1 and 2 g L −1 .