Preparation and evaluation of the effect of F e 3 O 4 @piroctone olamine magnetic nanoparticles on matrix metalloproteinase‐2: A preliminary in vitro study

In the present study, Fe 3 O 4 magnetic nanoparticles were synthesized by the coprecipitation of Fe 2+ and Fe 3+ ions and used as a nanocarrier for the production of piroctone‐olamine‐loaded Fe 3 O 4 nanoparticles (Fe 3 O 4 @ PO NP s). The nanocrystalline structure of the prepared iron oxide species was confirmed by the X ‐ray diffraction spectroscopy method. Particle size distribution analysis showed that the size of Fe 3 O 4 @ PO NP s was in the range of 5–55 nm. The magnetization curve of Fe 3 O 4 @ PO NP s (with saturation magnetization of 28.2 emu/g) confirmed its ferromagnetic property. Loading of PO on the surface of Fe 3 O 4 NP s qualitatively verified by Fourier transform infrared spectrum obtained from Fe 3 O 4 @ PO NP s. Cytotoxicity studies on the human fibrosarcoma cell line ( HT ‐1080) revealed higher inhibitory effect of Fe 3 O 4 @ PO NP s (50% cell death [ IC 50 ] of 8.1 µg/mL) as compared with Fe 3 O 4 NP s ( IC 50 of 117.1 µg/mL) and PO ( IC 50 of 71.2 µg/mL) alone. In the case of human normal fibroblast (Hs68), the viability percentage was found to be 75% in the presence of Fe 3 O 4 @ PO NP s (120 µg/mL). Gelatin zymography showed 17.2% and 34.6% inhibition of matrix metalloproteinase‐2 ( MMP ‐2) in the presence of Fe 3 O 4 @ PO and PO , respectively, at the same concentration of 40 µg/mL, whereas Fe 3 O 4 NP s did not inhibit MMP ‐2 at any concentration.