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Enhanced production of 2,3‐butanediol by overexpressing acetolactate synthase and acetoin reductase in K lebsiella pneumoniae
Author(s) -
Guo XueWu,
Zhang YunHui,
Cao ChunHong,
Shen Tong,
Wu MingYue,
Chen YeFu,
Zhang CuiYing,
Xiao DongGuang
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1217
Subject(s) - acetoin , 2,3 butanediol , lactose , acetolactate synthase , fermentation , klebsiella pneumoniae , sucrose , biochemistry , reductase , strain (injury) , chemistry , food science , microbiology and biotechnology , biology , enzyme , escherichia coli , anatomy , gene
Mutants with overexpression of α‐acetolactate synthase ( ALS ), α‐acetolactate decarboxylase, and acetoin reductase (AR), either individually or in combination, were constructed to improve 2,3‐butanediol (2,3‐ BD ) production in K lebsiella pneumoniae . The recombinant strains were characterized in terms of the enzyme activity, 2,3‐ BD yield, and expression levels. The recombinant K . pneumoniae strain ( KG ‐rs) that overexpressed both ALS and AR showed an improved 2,3‐ BD yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibited higher 2,3‐ BD productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2,3‐ BD production of KG ‐rs in a batch fermentation with glucose as the carbon source was 12% higher than that of the parental strain.