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Cellular redox homeostasis in endothelial cells treated with nonmodified and Fenton‐modified nanodiamond powders
Author(s) -
SolarskaŚciuk K.,
Gajewska A.,
Skolimowski J.,
Gajek A.,
Bartosz G.
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1208
Subject(s) - chemistry , oxidative stress , reactive oxygen species , cytotoxicity , antioxidant , apoptosis , nanoparticle , umbilical vein , glutathione , biophysics , nanodiamond , biochemistry , endothelial stem cell , diamond , microbiology and biotechnology , nanotechnology , enzyme , biology , materials science , in vitro , organic chemistry
Diamond nanoparticles find numerous applications in pharmacy, medicine, cosmetics, and biotechnology. However, possible adverse cellular effects of diamond nanoparticle cells have been reported, which may limit their use. The aim of this study was to compare the effect of nonmodified diamond nanoparticles ( D ) and diamond nanoparticles modified by the Fenton reaction ( D+OH ) on human umbilical cord endothelial cells ( HUVEC‐ST ). We found that both D and D+OH show time‐ and concentration‐dependent cytotoxicity, inducing apoptosis and necrosis of HUVEC‐ST . Interaction with D and D+OH also induced changes in the production of reactive oxygen and nitrogen species and changes in the level of glutathione and activities of antioxidant enzymes in the cells. These data demonstrate that diamond nanoparticles may induce oxidative stress in human endothelial cells, which contributes to their cytotoxic effects seen at higher concentrations of D and D+OH .