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Optimization of 2,3‐butanediol production by Enterobacter cloacae in simultaneous saccharification and fermentation of corncob residue
Author(s) -
Zhang CuiYing,
Peng XiaoPei,
Li Wei,
Guo XueWu,
Xiao DongGuang
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1198
Subject(s) - corncob , fermentation , enterobacter cloacae , chemistry , residue (chemistry) , xylose , xylitol , food science , hydrolysis , 2,3 butanediol , butanediol , urea , corn steep liquor , lactic acid , bacillus coagulans , biochemistry , bacteria , biology , raw material , organic chemistry , enterobacteriaceae , genetics , escherichia coli , gene
Corncob residue, a waste in xylose or xylitol production, was utilized to produce 2,3‐butanediol (2,3‐ BD ) via simultaneous saccharification and fermentation ( SSF ). This study developed the optimal conditions for production of 2,3‐BD by using a heat‐resistant strain, Enterobacter cloacae UV4, to perform SSF of the corncob residue. Urea, lactic acid, sodium citrate, and MgSO 4 , selected by the P lackett– B urman experiment, were determined to be significant independent variables to conduct the response surface experiment. With the optimized medium, a total production of 28.923 g/L for 2,3‐ BD and acetoin ( BA ) was obtained at 60 H. Furthermore, 43.162 g/L of BA production and 0.553 g/L/H of productivity were obtained by fed‐batch SSF , which was 0.424 g diol/g consumed corncob residue. The results suggest that the waste corncob residue could be used as an available substrate for the production of 2,3‐ BD by E. cloacae UV 4, as well as a potential resource to improve the economics of microbial compound production.