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Periostin induces chemoresistance in colon cancer cells through activation of the PI 3 K / A kt/survivin pathway
Author(s) -
Xiao Zhiming,
Wang Xiaoyan,
Wang Aimin
Publication year - 2015
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1193
Subject(s) - periostin , survivin , chemistry , propidium iodide , cancer research , gene silencing , microbiology and biotechnology , apoptosis , annexin , small interfering rna , cancer cell , biology , transfection , programmed cell death , cancer , biochemistry , gene , extracellular matrix , genetics
Abstract In the present study, we aimed to explore the effects of periostin, a cell adhesion protein, on chemoresistance in colon cancer cells. Reverse‐transcription polymerase chain reaction and W estern blot analyses were employed to detect periostin expression in SW 480 and HT ‐29 colon cancer cells treated with oxaliplatin or fluorouracil (5‐ FU ). Small interfering RNA was used to downregulate endogenous periostin. Annexin‐ V /propidium iodide staining was performed to analyze the effects of periostin on drug‐induced apoptosis. The results showed that treatment with oxaliplatin or 5‐ FU elevated both the m RNA and protein levels of periostin in SW 480 and HT ‐29 cells. Silencing of periostin significantly ( P < 0.01) augmented drug‐induced apoptosis in colon cancer cells, coupled with enhanced cleavage of caspase‐3 and poly( ADP ‐ribose) polymerase. Mechanistic studies revealed that periostin silencing significantly ( P < 0.01) suppressed the expression of survivin, an antiapoptotic protein in colon cancer cells. Enforced expression of survivin repressed drug‐induced apoptosis in periostin‐depleted SW 480 and HT ‐29 cells. Additionally, periostin overexpression increased the expression of survivin and the phosphorylation of A kt, which was reversed by pretreatment with the phosphatidylinositol 3‐kinase ( PI 3 K )‐specific inhibitor LY 294002. Taken together, our data demonstrate that periostin induces chemoresistance in colon cancer cells through activation of the PI 3 K / A kt/survivin pathway.