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R as/ PKA signal transduction pathway participates in the regulation of S accharomyces cerevisiae cell apoptosis in an acidic environment
Author(s) -
Lastauskienė Eglė,
Zinkevičienė Auksė,
Čitavičius Donaldas
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1183
Subject(s) - apoptosis , saccharomyces cerevisiae , programmed cell death , signal transduction , biology , microbiology and biotechnology , yeast , viability assay , cell , cell growth , biochemistry
The acidification of the medium is observed during yeast cell growth. This process contributes to the emission of organic acids, mainly acetic acid. Acetic acid is known as the inducer of apoptosis in the yeast S accharomyces cerevisiae . In this study, we showed that hydrochloric acid can also induce apoptosis in yeast cells, and the apoptotic phenotype triggered by treating yeast cells with hydrochloric acid is modulated by the R as/ PKA pathway. The R as/ PKA pathway is highly conserved between all eukaryotic organisms, as well as cell processes that are related to apoptosis and aging. In this research, we demonstrated that the activation of the R as/ PKA pathway by insertion of R as2 Val19 allele or deletion of PDE 2 gene increases cell death, displaying the markers of apoptosis in an acidic environment. Downregulation of the pathway by deletion of RAS 2, RAS 1 , PDE 1 , and insertion of the H a‐ras gene increases the cell viability and diminishes cell death with the apoptotic phenotypes. The deletion of PDE 1 gene and double deletion of both phosphodiesterase genes prevent the induction of apoptosis in the cells. Modulations in the R as/ PKA pathway affect cell viability and apoptosis during natural gradual acidification of the medium as well as in acid stress conditions.