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Engineering the MEP pathway enhanced ajmalicine biosynthesis
Author(s) -
Chang Kai,
Qiu Fei,
Chen Min,
Zeng Lingjiang,
Liu Xiaoqiang,
Yang Chunxian,
Lan Xiaozhong,
Wang Qiang,
Liao Zhihua
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1176
Subject(s) - biosynthesis , gene , transgene , metabolic engineering , biology , biochemistry , metabolic pathway , chemistry , microbiology and biotechnology
The 2‐ C ‐methyl‐ D ‐erythritol‐4‐phosphate ( MEP ) pathway genes encoding DXR and MECS from T axus species and STR from C atharanthus roseus were used to genetically modify the ajmalicine biosynthetic pathway in hairy root cultures of C . roseus . As expected, the STR ‐overexpressed root cultures showed twofold higher accumulation of ajmalicine than the control. It was important to discover that overexpression of the single DXR or MECS gene from the MEP pathway also remarkably enhanced ajmalicine biosynthesis in transgenic hairy root cultures, and this suggested that engineering the MEP pathway by overexpression of DXR or MECS promoted the metabolic flux into ajmalicine biosynthesis. The transgenic hairy root cultures with co‐overexpression of DXR and STR or MECS and STR had higher levels of ajmalicine than those with overexpression of a single gene alone such as DXR , MECS , and STR . It could be concluded that transgenic hairy root cultures harboring both DXR / MECS and STR possessed an increased flux in the terpenoid indole alkaloid biosynthetic pathway that enhanced ajmalicine yield, which was more efficient than cultures harboring only one of the three genes.