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Development of an immuno‐based colorimetric assay for white spot syndrome virus
Author(s) -
LoyprasertThananimit Suchera,
Saleedang Akrapon,
Deachamag Panchalika,
Waiyapoka Thanyaporn,
Neulplub Maitee,
Chotigeat Wilaiwan
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1169
Subject(s) - white spot syndrome , immunoassay , shrimp , virus , virology , streptavidin , alkaline phosphatase , microbiology and biotechnology , chemistry , chromatography , biology , enzyme , biochemistry , antibody , fishery , immunology , biotin
White spot syndrome virus ( WSSV ) is a major cause of infectious disease in cultured shrimp. A fast and reliable method for detecting and monitoring the amount of WSSV during farming would be extremely useful. This work describes a sandwich immunoassay that uses anti‐ GST ‐ VP 26, a WSSV ‐binding protein ( WBP ), and modified streptavidin magnesphere paramagnetic particles ( SMPP s) to develop the technique. The WBP was immobilized on SMPP s and later bound to different copies of WSSV . The binding was detected using anti‐ GST ‐ VP 26 conjugated to alkaline phosphatase. This enzymatic reaction successfully changed the test solution to a concentration‐dependent yellow color that was measured at 405 nm. The sensitivity of this method was between 1.6 × 10 4 and 1.6 × 10 7 copies µL −1 of WSSV . In this study, the color for detection and semiquantitative analysis is easily observed and measured and can lead to the development of a test kit for screening WSSV during shrimp farming.