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Adaptation of Dekkera bruxellensis to lignocellulose‐based substrate
Author(s) -
Tiukova Ievgeniia A.,
Barros Pita Will,
Sundell David,
Haddad Momeni Majid,
Horn Svein Jarle,
Ståhlberg Jerry,
Morais Marcos Antonio,
Passoth Volkmar
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1145
Subject(s) - hydrolysate , saccharomyces cerevisiae , yeast , ethanol fuel , ethanol , food science , alcohol dehydrogenase , biochemistry , fermentation , furfural , adaptation (eye) , biology , chemistry , hydrolysis , neuroscience , catalysis
Abstract Adaptation of Dekkera bruxellensis to lignocellulose hydrolysate was investigated. Cells of D. bruxellensis were grown for 72 and 192 H in batch and continuous culture, respectively (adapted cells). Cultivations in semisynthetic medium were run as controls (nonadapted cells). To test the adaptation, cells from these cultures were reinoculated in the lignocellulose medium, and growth and ethanol production characteristics were monitored. Cells adapted to lignocellulose hydrolysate had a shorter lag phase, grew faster, and produced a higher ethanol concentration as compared with nonadapted cells. A stability test showed that after cultivation in rich medium, cells partially lost the adapted phenotype but still showed faster growth and higher ethanol production as compared with nonadapted cells. Because alcohol dehydrogenase genes have been described to be involved in the adaptation to furfural in Saccharomyces cerevisiae , an analogous mechanism of adaptation to lignocelluloses hydrolysate of D. bruxellensis was hypothesized. However, gene expression analysis showed that genes homologous to S. cerevisiae ADH1 were not involved in the adaptation to lignocelluloses hydrolysate in D. bruxellensis .