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Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with ds RNA of natural origin
Author(s) -
Veinalde Rūta,
Petrovska Ramona,
Brūvere Rūta,
Feldmane Guna,
Pjanova Dace
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1143
Subject(s) - chemokine , cytokine , ex vivo , biology , proinflammatory cytokine , tumor necrosis factor alpha , immune system , immunology , macrophage inflammatory protein , interferon , peripheral blood mononuclear cell , innate immune system , inflammation , in vivo , in vitro , biochemistry , microbiology and biotechnology
Double‐stranded RNA (ds RNA ) is a pathogen‐associated molecular pattern, known for its ability to induce antiviral response and enhance communication between cells mediating innate and adaptive immune responses. The aim of this study was to characterize the effect of the ds RNA ‐containing product L arifan on the production of a wide spectrum of cytokines and chemokines in ex vivo cultivated peripheral blood mononuclear cells. Concentrations of 29 different cytokines were detected by a L uminex® 200™ S ystem using three M illiplex MAP M ultiplex A ssay K its. L arifan caused strong induction of chemokine macrophage inflammatory protein 1 β , I‐309, and TARC , proinflammatory cytokines IL ‐6, tumor necrosis factor ‐ α , granulocyte macrophage colony‐stimulating factor, anti‐inflammatory IL ‐10, and cellular immunity mediating factors IL ‐23 and interferon‐ γ . Considerable suppression of IL ‐16 and chemokine stromal cell‐derived factor 1 a+b and interferon gamma‐induced protein 10 was also observed. The network of molecules responding to the presence of L arifan revealed the pleiotropic effect this product exerts on immune response.