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Human umbilical cord–derived mesenchymal stem cells can secrete insulin in vitro and in vivo
Author(s) -
Niki Boroujeni Zahra,
Aleyasin Ahmad
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1127
Subject(s) - pdx1 , insulin , medicine , mesenchymal stem cell , endocrinology , stem cell , umbilical cord , glut2 , biology , chemistry , microbiology and biotechnology , immunology , glucose transporter , islet
Diabetes mellitus is characterized by autoimmune destruction of pancreatic beta cells, leading to decreased insulin production. Differentiation of mesenchymal stem cells ( MSC s) into insulin‐producing cells offers novel ways of diabetes treatment. MSC s can be isolated from the human umbilical cord tissue and differentiate into insulin‐secreting cells. Human umbilical cord–derived stem cells (h UDSC s) were obtained after birth, selected by plastic adhesion, and characterized by flow cytometric analysis. h UDSC s were transduced with nonintegrated lentivirus harboring PDX 1 (nonintegrated LV ‐ PDX 1) and was cultured in differentiation medium in 21 days. Pancreatic duodenum homeobox protein‐1 ( PDX 1) is a transcription factor in pancreatic development. Significant expressions of PDX 1, neurogenin3 (Ngn3), glucagon, glucose transporter2 (Glut2), and somatostatin were detected by quantitative RT ‐ PCR ( P < 0.05). PDX 1 and insulin proteins were shown by immunocytochemistry analysis. Insulin secretion of h UDSC s PDX1+ in the high‐glucose medium was 1.8 μU/mL. They were used for treatment of diabetic rats and could decrease the blood glucose level from 400 mg/dL to a normal level in 4 days. In conclusion, our results demonstrated that h UDSC s are able to differentiate into insulin‐producing cells by transduction with nonintegrated LV ‐ PDX 1. These h UDSC s PDX1+ have the potential to be used as a viable resource in cell‐based gene therapy of type 1 diabetes.