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Polymer‐based alternative method to extract bromelain from pineapple peel waste
Author(s) -
Lencastre Novaes Letícia Celia,
Carvalho Santos Ebinuma Valéria,
Mazzola Priscila Gava,
Júnior Adalberto Pessoa
Publication year - 2013
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1121
Subject(s) - bromelain , ananas , peg ratio , extraction (chemistry) , bromeliaceae , polyethylene glycol , proteolytic enzymes , chemistry , food science , chromatography , microbiology and biotechnology , protease , pulp and paper industry , enzyme , biochemistry , botany , biology , finance , economics , engineering
Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple ( Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two‐phase system formed by polyethylene glycol ( PEG ) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/ PEG ‐rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.