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Effects of magnetic nanoparticle‐incorporated human bone marrow–derived mesenchymal stem cells exposed to pulsed electromagnetic fields on injured rat spinal cord
Author(s) -
Cho Hyunjin,
Choi YunKyong,
Lee Dong Heon,
Park Hee Jung,
Seo YoungKwon,
Jung Hyun,
Kim SooChan,
Kim SungMin,
Park JungKeug
Publication year - 2013
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1109
Subject(s) - mesenchymal stem cell , human bone , spinal cord , bone marrow , magnetic nanoparticles , spinal cord injury , nanoparticle , anatomy , medicine , biomedical engineering , pathology , biology , materials science , neuroscience , nanotechnology , in vitro , biochemistry
Transplanting mesenchymal stem cells into injured lesions is currently under study as a therapeutic approach for spinal cord injury. In this study, the effects of a pulsed electromagnetic field ( PEMF ) on injured rat spinal cord were investigated in magnetic nanoparticle ( MNP )‐incorporated human bone marrow–derived mesenchymal stem cells (h BM ‐ MSC s). A histological analysis revealed significant differences in MNP ‐incorporated cell distribution near the injured site under the PEMF in comparison with that in the control group. We confirmed that MNP ‐incorporated cells were widely distributed in the lesions under PEMF . The results suggest that MNP ‐incorporated h BM ‐ MSC s were guided by the PEMF near the injured site, and that PEMF exposure for 8 H per day over 4 weeks promoted behavioral recovery in spinal cord injured rats. The results show that rats with MNP ‐incorporated h BM ‐ MSC s under a PEMF were more effective on the B asso, B eattie, and B resnahan behavioral test and suggest that the PEMF enhanced the action of transplanted cells for recovery of the injured lesion.