Premium
The combination of DHEA, histamine, and insulin increases adipogenic differentiation and enhances tissue transplantation outcome in mice
Author(s) -
Park Yoorim,
Jung Min Kyung,
Yoon Sun Young,
Lee HaReum,
Hur Dae Young,
Kim Daejin,
Yang Yoolhee,
Kim Tae Sung,
Kim Seonghan,
Yoon Suk Ran,
Park Hyun Jeong,
Bang Sa Ik,
Cho Dae Ho
Publication year - 2013
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1100
Subject(s) - adipose tissue , adipogenesis , medicine , transplantation , endocrinology , stem cell , adiponectin , cell adhesion , cellular differentiation , microbiology and biotechnology , 3t3 l1 , chemistry , biology , insulin , cell , insulin resistance , biochemistry , gene
Adipose stem cells (ASCs) are pluripotent cells that can generate pure fat tissue for regeneration. Differentiated adipose cells have been generated by a common inducer cocktail composed of dexamethasone, insulin, and isobutylmethylxanthine (DIM). The major drawbacks of adipose cells are their tendency to float on the culture media and their cost. To overcome some of these disadvantages, a new inducer cocktail that includes insulin, dehydroepiandrosterone, and histamine (D H IH) was tested. As a result, lipid accumulation was elevated more than twofold with D H IH than with DIM. Cell adhesion and viability, which are important factors for stable differentiation, were increased with D H IH and were proven through measurement of mRNA expression levels of adhesion marker genes, N‐cadherin and vascular cell adhesion molecule, as well as through an alamar blue assay. The expression of adipogenesis‐related genes, adiponectin, and glucose transporter type 4 lasted for a long time. To improve the efficiency of grafting, cell adhesion and neovascularization need to be increased. Neovascularization was observed around the transplanted adipose cells, which showed a higher number of vessel formation in D H IH than in DIM. The above results suggest that D H IH can produce pure differentiated adipose cells effectively and enhance their adhesion onto the target location when these differentiated adipose cells were applied as a clinical resource.