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Desulfurization activity and reusability of magnetite nanoparticle–coated Rhodococcus erythropolis FMF and R. erythropolis IGTS8 bacterial cells
Author(s) -
Bardania Hassan,
Raheb Jamshid,
MohammadBeigi Hossein,
Rasekh Behnam,
Arpanaei Ayyoob
Publication year - 2013
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1090
Subject(s) - nanoparticle , magnetite , coprecipitation , flue gas desulfurization , nuclear chemistry , sulfur , rhodococcus , chemical engineering , chemistry , materials science , nanotechnology , inorganic chemistry , organic chemistry , metallurgy , enzyme , engineering
The application of Fe 3 O 4 nanoparticles to the separation of desulfurizing bacterial cells and their influence on the desulfurization activity and reusability of the two bacterial strains Rhodococcus erythropolis FMF and R. erythropolis IGTS8 were investigated. Magnetite nanoparticles were synthesized via the reverse coprecipitation method. Transmission electron microscopy (TEM) images showed that the magnetite nanoparticles had sizes of 5.35 ± 1.13 (F1 nanoparticles) and 8.74 ± 1.18 nm (F2 nanoparticles) when glycine was added during the synthesis of nanoparticles and when it was absent from the reaction mixture, respectively. Glycine was added after the synthesis of both F1 and F2 nanoparticles to stabilize the nanoparticle dispersion. TEM images of cells treated with magnetite nanoparticles indicated that F1 nanoparticles were immobilized on the surface of bacterial cells more evenly than the F2 nanoparticles. Desulfurization activities of the F1 magnetite nanoparticle–coated R. erythropolis FMF and R. erythropolis IGTS8 cells (with sulfur‐removal percentage values of 70 ± 4 and 73 ± 3, respectively), as examined with the spectrophotometric Gibbs assay (based on dibenzothiophene degradation and sulfur‐removal percentage), were not significantly different from those for the free bacterial cells (67 ± 3 and 69 ± 4, respectively). These results indicate that magnetite nanoparticles cannot affect the desulfurization activity of cells examined in this work. Isolation of bacterial cells from the suspension using a magnet and evaluation of desulfurization activity of separated cells showed that Fe 3 O 4 nanoparticles can provide a high‐efficiency recovery of bacterial cells from a suspension, with the reused magnetite nanoparticle–coated bacterial cells being able to maintain their desulfurization activity efficiently.