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Characterization of pyrene biodegradation by white‐rot fungus Polyporus sp. S133
Author(s) -
Hadibarata Tony,
Kristanti Risky Ayu,
Fulazzaky Mohamad Ali,
Nugroho Agung Endro
Publication year - 2012
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1048
Subject(s) - polyporus , pyrene , gentisic acid , chemistry , biodegradation , laccase , dioxygenase , organic chemistry , lignin , cometabolism , fungus , chromatography , biochemistry , botany , bioremediation , bacteria , biology , enzyme , genetics , salicylic acid
A white‐rot fungus of Polyporus sp. S133 was isolated from an oil‐polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30‐day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy‐one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2‐dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2‐dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2‐dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1‐hydroxypyrene and gentisic acid. The structure of 1‐hydroxypyrene and gentisic acid was determined by gas chromatography–mass spectrometry after identification using thin‐layer chromatography.