Premium
Microbioreactors to manipulate oxygen tension and shear stress in the microenvironment of vascular stem and progenitor cells
Author(s) -
Abaci Hasan E.,
Devendra Raghavendra,
Soman Rohan,
Drazer German,
Gerecht Sharon
Publication year - 2012
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1010
Subject(s) - progenitor cell , umbilical vein , embryonic stem cell , microbiology and biotechnology , shear stress , stem cell , endothelial stem cell , oxygen tension , biology , progenitor , shear (geology) , chemistry , anatomy , oxygen , materials science , biochemistry , in vitro , paleontology , organic chemistry , gene , composite material
The dynamics of dissolved oxygen (DO) and shear stress in the vasculature microenvironment play a major role in determining the fate of stem cells in adults and during early embryonic development. In this study, we present a microbioreactor (MBR) that provides independent control over oxygen tension and shear stress in cultures of stem and progenitor cell types. We first describe the design principles and use a model‐driven approach for the optimization of the MBR geometry and operating conditions prior to its fabrication and assembly. We then demonstrate the utilization of the MBR for culturing adult human endothelial progenitors, human umbilical vein endothelial cells, and human embryonic stem cell‐derived smooth muscle cells under different DO and shear stress levels.