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An automated and high‐throughput method for adenosine triphosphate quantification
Author(s) -
Secka Fatou,
Allward Nicole E.,
Stoddart Amina K.,
Gag Graham A.
Publication year - 2020
Publication title -
awwa water science
Language(s) - English
Resource type - Journals
ISSN - 2577-8161
DOI - 10.1002/aws2.1202
Subject(s) - adenosine triphosphate , biomass (ecology) , water quality , environmental science , contamination , throughput , biochemical engineering , microorganism , computer science , chemistry , bacteria , biology , ecology , engineering , biochemistry , wireless , telecommunications , genetics
Exposure to microbial contamination through drinking water is a major global health concern. Effective management of microbial drinking water quality requires rapid detection equipment. Currently, microbial quality is monitored using time‐consuming laboratory methods, which delay any response. This study demonstrates the development of an automated and high‐throughput method for the measurement of viable biomass in water through the quantification of cellular adenosine triphosphate (ATP). The developed method was able to efficiently and accurately quantify cellular ATP in multiple water samples simultaneously. In addition, it proved to be 5× faster and as accurate as the Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water (ASTM D4012). The developed method has the potential to represent a significant advancement for microbial monitoring and could benefit utilities interested in measuring viable biomass in water to monitor the health of biofilters and the effectiveness of disinfection strategies.