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Improved cell culture immunofluorescent assay for detection of infectious Cryptosporidium spp. in prefinished water
Author(s) -
Alderisio Kerri A.,
McDonald Lisa A.,
Gabel Kurt W.,
Archuleta Joy,
Di Giovanni George D.
Publication year - 2019
Publication title -
awwa water science
Language(s) - English
Resource type - Journals
ISSN - 2577-8161
DOI - 10.1002/aws2.1126
Subject(s) - cryptosporidium , cryptosporidium parvum , flow cytometry , biology , microbiology and biotechnology , matrix (chemical analysis) , veterinary medicine , chromatography , chemistry , medicine , feces
The New York City Department of Environmental Protection's (NYC) Hillview Reservoir is an uncovered prefinished water storage reservoir postultraviolet (post‐UV) light disinfection. As an additional tool to the detection of total (viable and nonviable) oocysts using Methods 1623/1623.1, the performance of an improved cell culture immunofluorescent assay (CC‐IFA) for the specific detection of infectious oocysts in this NYC reservoir matrix was evaluated. Mean CC‐IFA matrix spike recovery of infectious Cryptosporidium parvum and Cryptosporidium hominis oocysts was comparable to the mean Method 1623 recovery of total oocysts, ranging from 29 to 46%. Experiments using ≤5 C. parvum or ≤10 C. hominis viable oocyst spikes prepared using flow cytometry indicated that the reservoir matrix did not adversely affect CC‐IFA detection. Therefore, the improved CC‐IFA appears useful for the detection of environmentally relevant, low numbers of infectious oocysts in the reservoir matrix and for the refined assessment of Cryptosporidium risk.