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Genome‐wide Association Study of Autism Spectrum Disorder in the East Asian Populations
Author(s) -
Liu Xiaoxi,
Shimada Takafumi,
Otowa Takeshi,
Wu YuYu,
Kawamura Yoshiya,
Tochigi Mamoru,
Iwata Yasuhide,
Umekage Tadashi,
Toyota Tomoko,
Maekawa Motoko,
Iwayama Yoshimi,
Suzuki Katsuaki,
Kakiuchi Chihiro,
Kuwabara Hitoshi,
Kano Yukiko,
Nishida Hisami,
Sugiyama Toshiro,
Kato Nobumasa,
Chen ChiaHsiang,
Mori Norio,
Yamada Kazuo,
Yoshikawa Takeo,
Kasai Kiyoto,
Tokunaga Katsushi,
Sasaki Tsukasa,
Gau Susan ShurFen
Publication year - 2016
Publication title -
autism research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.656
H-Index - 66
eISSN - 1939-3806
pISSN - 1939-3792
DOI - 10.1002/aur.1536
Subject(s) - transmission disequilibrium test , genetics , single nucleotide polymorphism , candidate gene , biology , autism spectrum disorder , autism , genetic association , genome wide association study , snp , copy number variation , gene , genotype , genome , medicine , psychiatry
Autism spectrum disorder is a heterogeneous neurodevelopmental disorder with strong genetic basis. To identify common genetic variations conferring the risk of ASD, we performed a two‐stage genome‐wide association study using ASD family and healthy control samples obtained from East Asian populations. A total of 166 ASD families ( n = 500) and 642 healthy controls from the Japanese population were used as the discovery cohort. Approximately 900,000 single nucleotide polymorphisms (SNPs) were genotyped using Affymetrix Genome‐Wide Human SNP array 6.0 chips. In the replication stage, 205 Japanese ASD cases and 184 healthy controls, as well as 418 Chinese Han trios ( n = 1,254), were genotyped by TaqMan platform. Case–control analysis, family based association test, and transmission/disequilibrium test (TDT) were then conducted to test the association. In the discovery stage, significant associations were suggested for 14 loci, including 5 known ASD candidate genes: GPC6 , JARID2 , YTHDC2 , CNTN4 , and CSMD1 . In addition, significant associations were identified for several novel genes with intriguing functions, such as JPH3, PTPRD, CUX1 , and RIT2 . After a meta‐analysis combining the Japanese replication samples, the strongest signal was found at rs16976358 ( P = 6.04 × 10 −7 ), which is located near the RIT2 gene. In summary, our results provide independent support to known ASD candidate genes and highlight a number of novel genes warranted to be further investigated in a larger sample set in an effort to improve our understanding of the genetic basis of ASD. Autism Res 2016, 9: 340–349 . © 2015 International Society for Autism Research, Wiley Periodicals, Inc.