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Efficient Design to Monitor the Site‐specific Sustained Release of a Non‐Emissive Anticancer Drug
Author(s) -
Venu Parvathy,
Le TrongNghia,
Kumar Pawan,
Patra Diptendu,
Kumar Rajan,
Lee ChengKang,
Rao N. Vijayakameswara,
Shunmugam Raja
Publication year - 2021
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.202100355
Subject(s) - norbornene , romp , polyethylene glycol , polymer chemistry , monomer , polymerization , chemistry , ring opening metathesis polymerisation , methacrylamide , copolymer , polymer , materials science , organic chemistry , metathesis , acrylamide
Abstract A pH‐responsive smart nanocarrier with significant components was synthesized by conjugating the non‐emissive anticancer drug methyl orange and polyethylene glycol derived folate moiety to the backbone of polynorbornene. Complete synthesis procedure and characterization methods of three monomers included in the work: norbornene‐derived Chlorambucil (Monomer 1), norbornene grafted with polyethylene glycol, and folic acid (Monomer 2) and norbornene attached methyl orange (Monomer 3) connected to the norbornene backbone through ester linkage were clearly discussed. Finally, the random copolymer CHO PEG FOL METH was synthesized by ring‐opening metathesis polymerization (ROMP) using Grubbs′ second‐generation catalyst. Advanced polymer chromatography (APC) was used to find the final polymer‘s molecular weight and polydispersity index (PDI). Dynamic light scattering, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were utilized to explore the prodrug‘s size and morphology. Release experiments of the anticancer drug, Chlorambucil and the coloring agent, methyl orange, were performed at different pH and time. Cell viability assay was carried out for determining the rate of survived cells, followed by the treatment of our final polymer named CHO PEG FOL METH.