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Detection of Urinary Albumin Using a “Turn‐on” Fluorescent Probe with Aggregation‐Induced Emission Characteristics
Author(s) -
Hu Qi,
Yao Bicheng,
Owyong Tze Cin,
Prashanth Sharon,
Wang Changyu,
Zhang Xinyi,
Wong Wallace W. H.,
Tang Youhong,
Hong Yuning
Publication year - 2021
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.202100180
Subject(s) - human serum albumin , fluorescence , chemistry , urinalysis , linear range , urine , analytical chemistry (journal) , chromatography , detection limit , optics , biochemistry , physics
Human serum albumin (HSA) is a broadly used biomarker for the diagnosis of various diseases such as chronic kidney disease. Here, a fluorescent probe TC426 with aggregation‐induced emission (AIE) characteristics is reported as a sensitive and specific probe for HSA. This probe is non‐emissive in aqueous solution, meanwhile it shows bright fluorescence upon interacting with HSA, which makes it applicable in detecting HSA with a high signal to noise ratio. Besides, the fluorescence of TC426 exhibits a high linear correlation with the concentration of albumin in the range of microalbumin (20–200 mg/L), which has a significant importance for the early diagnosis of glomerulus related diseases. Compared with previously reported HSA probes TPE‐4TA and BSPOTPE , TC426 shows comparable anti‐interference ability towards creatinine and other major components in urine but is excited by a longer excitation wavelength at the visible light range. Finally, with the established assay, TC426 shows excellent performance in detecting HSA in real human urine, indicating its great potential in practical urinalysis.