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Effective Separation of Human Milk Glycosides using Carbon Dioxide Supercritical Fluid Chromatography
Author(s) -
Liou ShihWei,
Fang JiaLin,
Lin HungWei,
Tsai TengWei,
Huang HsinHui,
Liang ChinYu,
Yang ChengRuel,
Wei GuorTzo,
Yu ChingChing
Publication year - 2021
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.202001404
Subject(s) - chemistry , structural isomer , supercritical fluid chromatography , chromatography , glycan , carbohydrate , glycoside , supercritical carbon dioxide , gas chromatography , organic chemistry , extraction (chemistry) , biochemistry , glycoprotein
Carbohydrate purification remains problematic due to the intrinsic diversity of structural isomers present in nature. Although liquid chromatography‐based techniques are suitable for analyzing or preparing most glycan structures acquired either from natural sources or through chemical or enzymatic synthesis, the separation of regioisomers or linkage isomers with a clear resolution remains challenging. Herein, a carbon dioxide supercritical fluid chromatography (SFC) method was devised to resolve 18 human milk glycosides: oligomers (disaccharides to hexasaccharides), fucosylated regioisomers (lacto‐ N ‐fucopentaose I, III, and V; lacto‐ N ‐neofucopentaose V; lacto‐ N ‐difucohexaose III; blood group H 1 antigen; and TF‐LNnT), and connectivity isomers (lacto‐ N ‐tetraose/lacto‐ N ‐neotetraose and para ‐lacto‐ N ‐hexaose/ para ‐lacto‐ N ‐neohexaose/type‐1 hexasaccharide). The analysis of these glycosides represents a major limitation associated with conventional carbohydrate analysis. The unprecedented resolution achieved by the SFC method indicates the suitability of this key technology for revealing complex human milk glycomes.