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Duplex‐forming Oligonucleotide of Triazole‐linked RNA
Author(s) -
Fujino Tomoko,
Suzuki Takeru,
Ooi Tsugumi,
Ikemoto Koki,
Isobe Hiroyuki
Publication year - 2019
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201901112
Subject(s) - duplex (building) , oligonucleotide , rna , computational biology , biology , genetics , dna , gene
An oligonucleotide of triazole‐linked RNA ( TL RNA) was synthesized by performing consecutive copper‐catalyzed azide‐alkyne cycloaddition reactions for elongation. The reaction conditions that had been optimized for the synthesis of 3‐mer TL RNA were found to be inappropriate for longer oligonucleotides, and the conditions were reoptimized for the solid‐phase synthesis of an 11‐mer TL RNA oligonucleotide. Duplex formation of the 11‐mer TL RNA oligonucleotide was examined with the complementary oligonucleotide of natural RNA to reveal the effects of the 2′‐OH groups on the duplex stability.