Premium
Degradation of Perfluorooctane Sulfonamide by Acinetobacter Sp. M and Its Extracellular Enzymes
Author(s) -
Hao Jian,
Wang Penghong,
Kang Yufei,
He Haitao,
Luo Huihua,
Kim Sarah,
Niu Lili,
Jiang Haizhen,
Ma Kesen
Publication year - 2019
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201900638
Subject(s) - strain (injury) , perfluorooctane , degradation (telecommunications) , acinetobacter , enzyme , chemistry , biodegradation , extracellular , carbon fibers , biochemistry , organic chemistry , biology , materials science , sulfonate , telecommunications , antibiotics , anatomy , composite number , computer science , composite material , sodium
The Acinetobacter sp. strain M isolated from a contaminated soil sample in Jiangsu Province of China was found to be able to degrade perfluorooctane sulfonamide (PFOSA) effectively. Fluoride anion (F − ) released from PFOSA degradation was detected by ion chromatography, and showed positive correlation to the growth curve of Acinetobacter sp. strain M. The PFOSA degradation efficiency of strain M was approximately 27 %, as assessed by GC analysis. It was shown that enzymes localized outside of cells of Acinetobacter sp. strain M catalyzed the degradation of PFOSA. This further indicates a possibly new (multi‐step/pathway) mechanism for PFOSA degradation. It revealed that the extracellular enzyme of the Acinetobacter strain M preferentially cleaves carbon‐carbon and carbon‐fluorine bonds instead of destroying the carbon‐sulfur bond. The growth condition for Acinetobacter sp. strain M was optimized at 30 °C and pH 7.0 in the presence of 2000 mg L −1 of PFOSA and 0.5 % (v/v) of Tween‐20. The optimal PFOSA degradation time was found to be 12 h, with a degradation efficiency of 76 % by extracellular enzymes in strain M as determined by GC analysis. The result may provide potential applications for biodegradition of perfluoro organic compounds, such as derivatives of perfluorooctane (C8).