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Front Cover: Strong Inhibitory Effects of Antisense Probes on Gene Expression through Ultrafast RNA Photocrosslinking (Chem. Asian J. 11/2019)
Author(s) -
Fujimoto Kenzo,
YangChun Hung,
Nakamura Shigetaka
Publication year - 2019
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201900417
Subject(s) - rna , gene expression , antisense rna , microbiology and biotechnology , gene , intracellular , chemistry , biophysics , biology , biochemistry
Ultrafast RNA photocrosslinking can regulate the intracellular gene expression via antisense effects using photocrosslinkable antisense oligodeoxynucleotides (pcASOs). The inhibitory effect of pcASOs on gene expression was dependent on the photocrosslinking rate of 3‐cyanovinylcarbazole with d ‐threoninol ( CNV D), CNV K, or psoralen. The ultrafast RNA photocrosslinking induced the formation of a thermally irreversible covalent bond between pcASOs and the target RNA. These results indicate a regulatory effect of the photocrosslinker and photoirradiation energy using pcASOs on the gene expression level. More information can be found in the Communication by Kenzo Fujimoto, Hung Yang‐Chun, and Shigetaka Nakamura on page 1912 in Issue 11, 2019 (DOI: 10.1002/asia.201801917).