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A Maleimide‐functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells
Author(s) -
Zhang Shouxiang,
Liu Mengjie,
Tan Lewis Yi Fong,
Hong Quentin,
Pow Ze Liang,
Owyong Tze Cin,
Ding Siyang,
Wong Wallace W. H.,
Hong Yuning
Publication year - 2019
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201900150
Subject(s) - proteostasis , maleimide , unfolded protein response , flow cytometry , fluorescence , chemistry , biophysics , confocal microscopy , fluorescence microscope , protein aggregation , microbiology and biotechnology , fluorescent protein , nanotechnology , green fluorescent protein , biochemistry , materials science , biology , endoplasmic reticulum , polymer chemistry , physics , quantum mechanics , gene
Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation of unfolded proteins, which has been found to associate with a number of disease conditions including neurodegenerative diseases, diabetes and inflammation. Here we report a maleimide‐functionalized tetraphenylethene (TPE)‐derivatized fluorescent dye, TPE‐NMI, which shows fluorescence turn‐on property upon reacting with unfolded proteins in vitro and in live cells under proteostatic stress conditions. The level of unfolded proteins can be measured by flow cytometry and visualized with confocal microscopy.