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Fluorescence Turn‐on Synthetic Lipid Rafts on Supramolecular Sheets and Hierarchical Concanavalin A Assembly
Author(s) -
Kim Yongju,
Liu Xin,
Li Huichang,
Lee Myongsoo
Publication year - 2019
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201900122
Subject(s) - lipid raft , supramolecular chemistry , raft , membrane , chemistry , concanavalin a , fluorescence , self assembly , biophysics , copolymer , crystallography , polymer , organic chemistry , crystal structure , biochemistry , physics , quantum mechanics , in vitro , biology
Here we report fluorescence turn‐on synthetic lipid rafts by self‐assembly of a cationic distyrylanthracene derivative on a negatively‐charged sheet in an aqueous solution. First, the negatively‐charged 2D membrane structure is formed by lateral associations of aromatic rods with carboxylate groups. Then, the synthetic rafts are floated on the surface of the negatively‐charged sheets through electrostatic interactions. The fluorescence of the synthetic rafts is turned on due to the aggregation of the positively‐charged AIE dye on the sheets, facilitating monitoring of the formation of rafts. Concanavalin A (Con A) protein can load hierarchically onto the synthetic rafts at neutral pH to provide discrete Con A aggregates with a uniform size of ≈12 nm. The uniform aggregates of Con A on the synthetic rafts can stimulate Jurkat cells with enhanced efficiency, as compared with random‐sized aggregates of Con A.