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Imaging Macrophage Phagocytosis Using AIE Luminogen‐Labeled E. coli
Author(s) -
Gu Meijia,
Zeng Zixuan,
Wu MingYu,
Leung JongKai,
Zhao Engui,
Wang Shidong,
Chen Sijie
Publication year - 2019
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201801859
Subject(s) - phagocytosis , internalization , bacteria , macrophage , chemistry , microbiology and biotechnology , fluorescence , biophysics , phagosome , biology , biochemistry , cell , in vitro , genetics , physics , quantum mechanics
Phagocytosis of bacteria is an important biological process. Gaining insight into this process may greatly benefit related pathological studies and further contribute to development of therapies for infectious diseases. Tools for studying these internalization processes, however, are limited. Herein, we demonstrate the feasibility of employing an environmentally sensitive aggregation‐induced emission (AIE) probe for bacteria labeling and imaging. By tracking the fluorescence variation of the stained bacteria, the pH changes of its microenvironment can be monitored. In this way, the phagocytic entry of these bacteria into the macrophage cells and the intravacuolar acidification can be visualized in real‐time.