Premium
Live Cell Imaging Using Photoswitchable Diarylethene‐Doped Fluorescent Polymer Dots
Author(s) -
Osakada Yasuko,
Fukaminato Tuyoshi,
Ichinose Yuma,
Fujitsuka Mamoru,
Harada Yoshie,
Majima Tetsuro
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201701038
Subject(s) - diarylethene , fluorescence , live cell imaging , materials science , nanomaterials , nanoparticle , nanotechnology , biological imaging , fluorescence lifetime imaging microscopy , photochromism , biophysics , photochemistry , chemistry , cell , optics , biochemistry , physics , biology
Fluorescence photoswitching using nanomaterials has recently emerged as a promising approach for the imaging of biological targets. However, despite intensive research efforts during the last decade, practical microscopy of biological targets using photoswitchable nanoparticles in real time remains challenging. To address this problem, we have developed live macrophage cell imaging and single particle imaging methods, using photoswitchable fluorescent diarylethene‐doped polymer nanoparticles (P‐dots) under Xe lamp irradiation. We established a 34‐times prolonged “off‐state”, using P‐dots doped with a diarylethene‐containing methoxy substituent, upon visible‐light irradiation using a Xe lamp and a green fluorescent protein filter cube. To demonstrate the practicality of doped P‐dots imaging, we imaged lysosomes in macrophage cells, and observed 11‐times slower recovery of the fluorescence from the “off‐state” to the “on‐state”, indicating their potential for cellular imaging.