Premium
Precise Labeling and Tracking of Lipid Droplets in Adipocytes Using a Luminescent ZnSalen Complex
Author(s) -
Tang Juan,
Zhang Yanfei,
Yin HaoYan,
Xu Guoheng,
Zhang JunLong
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201701010
Subject(s) - lipid droplet , luminescence , chemistry , tracking (education) , biophysics , biology , biochemistry , materials science , optoelectronics , psychology , pedagogy
Designing two‐photon probes for precise labeling of lipid droplets (LDs) and monitoring LD dynamics in adipocytes is of great significance to understand LD homeostasis. We herein report that a luminescent metal complex LD‐TPZn can specifically image LDs in adipose cells and tissue using one‐ or two‐photon fluorescence microscopy. Importantly, LD‐TPZn exhibited higher specificity to LDs than the commercial dyes Nile Red and Bodipy 493/503, probably due to different cellular uptake pathways, that is, clathrin‐mediated endocytosis and non‐selectively passive diffusion, respectively. More importantly, LD‐TPZn can be applied as a two‐photon LD probe to image adipose tissue, one of the most challengeable tissues for traditional one‐photon fluorescence microscope imaging due to the strong light scattering. Most importantly, LD‐TPZn can be used to monitor LD growth during adipogenesis of preadipocytes, which is highly desirable to unravel the relationship between LD homeostasis and metabolic diseases.