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Dual‐Emission Channels for Simultaneous Sensing of Cysteine and Homocysteine in Living Cells
Author(s) -
Li Ying,
Liu Weimin,
Zhang Hongyan,
Wang Mengqi,
Wu Jiasheng,
Ge Jiechao,
Wang Pengfei
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201700583
Subject(s) - cysteine , fluorescence , chemistry , homocysteine , coumarin , glutathione , derivative (finance) , photochemistry , biophysics , biochemistry , enzyme , organic chemistry , biology , physics , quantum mechanics , financial economics , economics
The development of a fluorescent probe to distinguish between cysteine (Cys) and homocysteine (Hcy) is always a challenge owing to their structural similarity, and the simultaneous detection of Cys and Hcy by utilizing different emission channels is especially difficult. In this work, we designed and synthesized a new fluorescent probe to differentiate between Cys and Hcy on the basis of a coumarin derivative with a chlorine atom and an α,β‐unsaturated aldehyde. Cys and Hcy induced different cascade reactions with the probe, which led to different products with distinct photophysical properties. The nonfluorescent probe responded to Cys and emitted strong blue fluorescence, whereas it reacted with Hcy and generated yellow fluorescence without interference from glutathione. In addition, the probe was successfully applied to distinguish between Cys and Hcy in living cells.