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Graphene Oxide Conjugated Magnetic Beads for RNA Extraction
Author(s) -
Pham XuanHung,
Baek Ahruem,
Kim Tae Han,
Lee Sang Hun,
Rho WonYeop,
Chung WooJae,
Kim DongEun,
Jun BongHyun
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201700554
Subject(s) - rna , rna extraction , conjugated system , lysis , graphene , chaotropic agent , extraction (chemistry) , magnetic separation , adsorption , chromatography , magnetic bead , magnetic nanoparticles , chemistry , oxide , materials science , nanotechnology , biochemistry , nanoparticle , organic chemistry , polymer , metallurgy , gene
A magnetic material that consists of silica‐coated magnetic beads conjugated with graphene oxide (GO) was successfully prepared for facile ribonucleic acid (RNA) extraction. When the GO‐modified magnetic beads were applied to separate the RNA from the lysed cell, the cellular RNAs were readily adsorbed to and readily desorbed from the surface of the GO‐modified magnetic beads by urea. The amount of RNA extracted by the GO‐modified magnetic beads was ≈170 % as much as those of the control extracted by a conventional phenol‐based chaotropic solution. These results demonstrate that the facile method of RNA separation by using GO‐modified magnetic beads as an adsorbent is an efficient and simple way to purify intact cellular RNAs and/or microRNA from cell lysates.