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A Carborane‐Containing Fluorophore as a Stain of Cellular Lipid Droplets
Author(s) -
Wu Andrew,
Kolanowski Jacek L.,
Boumelhem Badwi B.,
Yang Kylie,
Lee Rebecca,
Kaur Amandeep,
Fraser Stuart T.,
New Elizabeth J.,
Rendina Louis M.
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201700423
Subject(s) - fluorophore , lipid droplet , fluorescence , stain , biophysics , confocal microscopy , chemistry , fluorescence microscope , ex vivo , confocal , nile red , flow cytometry , microscopy , staining , biochemistry , in vitro , microbiology and biotechnology , biology , physics , geometry , mathematics , quantum mechanics , optics , genetics
The use of fluorescent markers and probes greatly enhances biological investigations but relies on the provision of an array of fluorophores with diverse properties. Herein we report a novel carborane‐containing coumarin, 5 , which is sufficiently lipophilic to localise in cellular lipid droplets. In non‐polar solvents which show comparable polarities to those of a lipid environment, compound 5 exhibits a fluorescence quantum yield two orders of magnitude greater than found in aqueous solvents, adding a further degree of selectivity to lipid droplet imaging. Compound 5 can stain lipid droplets in ex vivo adipocytes as well as in cultured cells, and can be utilised in flow cytometry as well as confocal microscopy.