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Systematic Study of Functionalizable, Non‐Biofouling Agarose Films with Protein and Cellular Patterns on Glass Slides
Author(s) -
Han Gyeongyeop,
Hong Daehwa,
Lee Bong Soo,
Ha EunRae,
Park Ji Hun,
Choi Insung S.,
Kang Sung Min,
Lee Jungkyu K.
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201700010
Subject(s) - agarose , biofouling , fluorescence microscope , materials science , aqueous solution , microcontact printing , sepharose , chemical engineering , fluorescence , chemistry , polymer chemistry , nanotechnology , chromatography , organic chemistry , biochemistry , optics , physics , engineering , membrane , enzyme
Herein we demonstrate a systematic investigation of chemically functionalizable, non‐biofouling agarose films over large‐area glass surfaces. Agarose films, prepared with various concentrations of aqueous agarose, were activated by using periodate oxidation to generate aldehyde groups at the termini of the agarose chains. The non‐biofouling efficacy and binding capabilities of the activated films were evaluated by using protein and cellular patterning, performed by using a microarrayer, microcontact printing, and micromolding in capillaries. Characterization by using a fluorescence slide scanner and a scanning‐probe microscope revealed that the pore sizes of the agarose films played an important role in achieving desirable film performance; the 0.2 wt % agarose film exhibited the optimum efficacy in this work.