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Luminescent Ruthenium(II) Polypyridyl Complexes as Nonviral Carriers for DNA Delivery
Author(s) -
Bhat Satish S.,
Revankar Vidyanand K.,
Khan Ayesha,
Pinjari Rahul V.,
Necas Marek
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201601515
Subject(s) - ruthenium , luminescence , chemistry , phenanthroline , circular dichroism , dna , dna condensation , photochemistry , spectroscopy , bipyridine , crystallography , transfection , materials science , organic chemistry , crystal structure , catalysis , biochemistry , physics , optoelectronics , quantum mechanics , gene
Abstract Two new luminescent ruthenium(II) polypyridyl complexes, [Ru(bpy) 2 (tpt‐phen)]Cl 2 ( 1 ; bpy=2,2′‐bipyridine, tpt‐phen=triptycenyl‐1,10‐phenanthroline) and [Ru(phen) 2 (tpt‐phen)]Cl 2 ( 2 ; phen=1,10‐phenanthroline), have been developed as potential nonviral vectors for DNA delivery. Photophysical and electrochemical properties of the complexes have been investigated and corroborated with electronic structure calculations. DNA condensation by these complexes has been investigated by UV/Vis and emission spectroscopy, circular dichroism spectroscopy, atomic force microscopy, dynamic light scattering, confocal microscopy, and electrophoretic mobility studies. These complexes interact with DNA and efficiently condense DNA into globular nanoparticles that are taken up efficiently by HeLa cells. DNA cleavage inability and biocompatibility of complexes have been explored. Both complexes have good gene transfection abilities.