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Turn‐On Colorimetric Platform for Dual Activity Detection of Acid and Alkaline Phosphatase in Human Whole Blood
Author(s) -
Hu Qiong,
Zhou Baojing,
Li Feng,
Kong Jinming,
Zhang Xueji
Publication year - 2016
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201601006
Subject(s) - alkaline phosphatase , ascorbic acid , chemistry , phosphatase , detection limit , colorimetry , biochemistry , human blood , substrate (aquarium) , whole blood , enzyme , chromatography , biology , food science , immunology , physiology , ecology
The activity detection of acid phosphatase (ACP) and alkaline phosphatase (ALP) is of great importance to the diagnosis and prognosis of related diseases. In this work, we report for the first time a turn‐on colorimetric platform for the activity detection of ACP and ALP, by exploiting Cu(BCDS) 2 2− (BCDS=bathocuproinedisulfonate) as the probe. The presence of ACP or ALP dephosphorylates the substrate ascorbic acid 2‐phosphate to produce ascorbic acid, which then reduces Cu(BCDS) 2 2− into Cu(BCDS) 2 3− , leading to a turn‐on spectral absorption at 484 nm and a dramatic color change of the solution from colorless to orange‐red. The underlying metal‐to‐ligand charge‐transfer mechanism has been demonstrated by quantum mechanical computations. This platform allows a rapid, sensitive readout of ACP and ALP activities within the dynamic range from 0 to 220 mU ml −1 . In addition, it is highly immune to false‐positive results and also highly selective. More importantly, it is applicable in the presence of human serum and even whole blood samples. These results demonstrate that our platform holds great potential in clinical practices and in the point‐of‐care analysis.