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Efficient Iterative Synthesis of O‐Acetylated Tri‐ to Pentadecasaccharides Related to the Lipopolysaccharide of Shigella flexneri Type 3 a through Di‐ and Trisaccharide Glycosyl Donors
Author(s) -
Hu Zhaoyu,
Bongat White Aileen F.,
Mulard Laurence A.
Publication year - 2017
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201600819
Subject(s) - shigella flexneri , glycosylation , trisaccharide , shigellosis , chemistry , shigella boydii , glycoside , hydrogenolysis , microbiology and biotechnology , polysaccharide , shigella sonnei , shigella , lipopolysaccharide , stereochemistry , escherichia coli , biochemistry , biology , immunology , gene , catalysis
Protection against bacterial infections, including shigellosis, can be achieved by antibodies against the bacterial surface polysaccharide. In line with our efforts to develop vaccine candidates for shigellosis, we report herein the synthesis of penta‐, deca‐, and pentadecasaccharides as well as tri‐, octa‐, and tridecasaccharides as the endchain and intrachain fragments, respectively, of the surface polysaccharide of Shigella flexneri 3 a, a prevalent serotype. The syntheses relied on the efficiency of the trichloroacetimidate glycosylation chemistry, whereby iteration with di‐ and trisaccharide building blocks provided fragments made of up to three mono‐O‐acetylated polysaccharide repeating units. Pd(OH) 2 ‐mediated hydrogenation/hydrogenolysis enabled the concomitant removal or conversion of up to 31 protecting groups of 4 different origins to provide the targets as propyl glycosides. Oligosaccharides comprising the octasaccharide segment were shown to display high conformational similarities in solution.