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Dual‐Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H 2 S
Author(s) -
Wei Chao,
Wang Runyu,
Zhang Changyu,
Xu Guoce,
Li Yanyan,
Zhang QiangZhe,
Li LuYuan,
Yi Long,
Xi Zhen
Publication year - 2016
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201600262
Subject(s) - fluorescence , hydrogen sulfide , chemistry , zebrafish , selectivity , dual (grammatical number) , endogeny , biophysics , biochemistry , biology , gene , catalysis , sulfur , physics , organic chemistry , quantum mechanics , art , literature
Hydrogen sulfide (H 2 S) is an important endogenous signaling molecule with a variety of biological functions. Development of fluorescent probes for highly selective and sensitive detection of H 2 S is necessary. We show here that dual‐reactable fluorescent H 2 S probes could react with higher selectivity than single‐reactable probes. One of the dual‐reactable probes gives more than 4000‐fold turn‐on response when reacting with H 2 S, the largest response among fluorescent H 2 S probes reported thus far. In addition, the probe could be used for high‐throughput enzymatic assays and for the detection of Cys‐induced H 2 S in cells and in zebrafish. These dual‐reactable probes hold potential for highly selective and sensitive detection of H 2 S in biological systems.