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Oxygen‐binding Protein Fiber and Microgel: Supramolecular Myoglobin–Poly(acrylate) Conjugates
Author(s) -
Ono Toshikazu,
Hisaoka Yasushi,
Onoda Akira,
Oohora Koji,
Hayashi Takashi
Publication year - 2016
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201501415
Subject(s) - myoglobin , heme , monomer , chemistry , acrylate , hemeprotein , polymer chemistry , supramolecular chemistry , polymer , linker , conjugated system , ligand (biochemistry) , crystallography , organic chemistry , biochemistry , crystal structure , operating system , receptor , computer science , enzyme
A supramolecular conjugate of myoglobin (Mb) and water‐soluble poly(acrylate), (PA 5k and PA 25k , where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme‐heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme‐propionates is coupled to the side‐chain carboxyl groups of poly(acrylate) activated by N ‐hydroxysuccinimide. The ratios of the heme‐modified monomer unit and the unmodified monomer unit ( m : n ) in the polymer chains of Heme‐PA 5k and Heme‐PA 25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb‐connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme‐modified polymer and dimeric apomyoglobin as a cross‐linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.