A 3,7‐Dihydroxyphenoxazine‐based Fluorescent Probe for Selective Detection of Intracellular Hydrogen Peroxide | Zendy

Han Zhiqiang | Zendy; Liang Xiao | Zendy; Ren Xuejiao | Zendy; Shang Luqing | Zendy; Yin Zheng | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

A 3,7‐Dihydroxyphenoxazine‐based Fluorescent Probe for Selective Detection of Intracellular Hydrogen Peroxide

Author(s) -

Han Zhiqiang,

Liang Xiao,

Ren Xuejiao,

Shang Luqing,

Yin Zheng

Publication year - 2016

Publication title -

chemistry – an asian journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.18

H-Index - 106

eISSN - 1861-471X

pISSN - 1861-4728

DOI - 10.1002/asia.201501304

Subject(s) - hydrogen peroxide , fluorescence , naked eye , detection limit , chemistry , reactive oxygen species , intracellular , selectivity , biophysics , photochemistry , biochemistry , chromatography , biology , catalysis , optics , physics

A novel N ‐borylbenzyloxycarbonyl‐3,7‐dihydroxyphenoxazine fluorescent probe (NBCD) for detecting H 2 O 2 in living cells is described. The probe could achieve high selectivity for detecting H 2 O 2 over other biological reactive oxygen species (ROS). In addition, upon addition of H 2 O 2 , NBCD exhibited color change from colorless to pink, which makes it a “naked‐eye” probe for H 2 O 2 detection. NBCD could not only be used to detect enzymatically generated H 2 O 2 but also to detect H 2 O 2 in living systems by using fluorescence spectroscopy, with a detection limit of 2 μ m . Importantly, NBCD enabled the visualization of epidermal growth factor (EGF)‐stimulated H 2 O 2 generation inside the cells.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research