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A 3,7‐Dihydroxyphenoxazine‐based Fluorescent Probe for Selective Detection of Intracellular Hydrogen Peroxide
Author(s) -
Han Zhiqiang,
Liang Xiao,
Ren Xuejiao,
Shang Luqing,
Yin Zheng
Publication year - 2016
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201501304
Subject(s) - hydrogen peroxide , fluorescence , naked eye , detection limit , chemistry , reactive oxygen species , intracellular , selectivity , biophysics , photochemistry , biochemistry , chromatography , biology , catalysis , optics , physics
A novel N ‐borylbenzyloxycarbonyl‐3,7‐dihydroxyphenoxazine fluorescent probe (NBCD) for detecting H 2 O 2 in living cells is described. The probe could achieve high selectivity for detecting H 2 O 2 over other biological reactive oxygen species (ROS). In addition, upon addition of H 2 O 2 , NBCD exhibited color change from colorless to pink, which makes it a “naked‐eye” probe for H 2 O 2 detection. NBCD could not only be used to detect enzymatically generated H 2 O 2 but also to detect H 2 O 2 in living systems by using fluorescence spectroscopy, with a detection limit of 2 μ m . Importantly, NBCD enabled the visualization of epidermal growth factor (EGF)‐stimulated H 2 O 2 generation inside the cells.