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Benzothiazole‐Pyimidine‐Based BF 2 Complex for Selective Detection of Cysteine
Author(s) -
Liu Qingsong,
Zhang Changli,
Wang Xiaoqing,
Gong Shuwen,
He Weijiang,
Liu Zhipeng
Publication year - 2016
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201501013
Subject(s) - benzothiazole , cysteine , chemistry , organic chemistry , enzyme
Due to the similar structure and reactivity of cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), the simultaneous discrimination of Cys over Hcy and GSH by a single fluorescent sensor is still a great challenge. In this work, a benzothiazole‐pyimidine‐based boron difluoride complex ( BPB ) was developed as a new fluorescent sensor for Cys. The sensor exhibits a highly selective “turn‐on” response to cysteine over Hcy, GSH and other amino acids in aqueous solution at physiological pH. The observed pseudo‐first‐order rate constant for the reaction of BPB with Cys was calculated to be about 0.062 min −1 . The detection limit of this sensor for Cys was determined to be 332 n m, and bioimaging of exogenous Cys by this sensor was successfully applied in living cells, thus indicating that this sensor holds great potential for biological applications.