Premium
Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference
Author(s) -
Fujino Tomoko,
Kogashi Kanako,
Okada Koudai,
Mattarella Martin,
Suzuki Takeru,
Yasumoto Kenichi,
Sogawa Kazuhiro,
Isobe Hiroyuki
Publication year - 2015
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201500765
Subject(s) - phosphoramidite , linker , oligonucleotide , rna , click chemistry , triazole , solid phase synthesis , combinatorial chemistry , oligonucleotide synthesis , chemistry , rna interference , biochemistry , computer science , peptide , organic chemistry , dna , gene , operating system
Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution‐phase click chemistry and solid‐phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click‐coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well‐established phosphoramidite method for the elongation. The series of chimeric 21‐mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small‐interfering RNA molecules.