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Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide‐Based Chemosensing Ensemble
Author(s) -
Meng Qingtao,
Jia Hongmin,
Gao Xue,
Wang Yue,
Zhang Run,
Wang Renjie,
Zhang Zhiqiang
Publication year - 2015
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201500690
Subject(s) - fluorophore , fluorescence , histidine , fluorescence microscope , chemistry , detection limit , titration , flow cytometry , quenching (fluorescence) , microscopy , metal ions in aqueous solution , aqueous solution , fluorescence lifetime imaging microscopy , photochemistry , nuclear chemistry , ion , chromatography , biochemistry , inorganic chemistry , biology , organic chemistry , microbiology and biotechnology , physics , amino acid , quantum mechanics , optics
We described a new ensemble‐approach‐based chemosensor, NCH‐Cu 2+ , for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu 2+ ions over other metal ions, accompanied with a 92.2 % fluorescence quenching. The decomplexation of NCH‐Cu 2+ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH‐Cu 2+ towards His showed a good linearity with a detection of limit at 70 n m . Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH‐Cu 2+ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH‐Cu 2+ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.