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Enzymatic Synthesis of a DNA Triblock Copolymer that is Composed of Natural and Unnatural Nucleotides
Author(s) -
Mitomo Hideyuki,
Watanabe Yukie,
Matsuo Yasutaka,
Niikura Kenichi,
Ijiro Kuniharu
Publication year - 2015
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201403108
Subject(s) - klenow fragment , copolymer , polymerization , nucleotide , dna , dna polymerase i , exonuclease , exonuclease iii , sticky and blunt ends , chemistry , template , combinatorial chemistry , dna polymerase , polymer , materials science , nanotechnology , base pair , biochemistry , polymerase chain reaction , organic chemistry , gene , escherichia coli , reverse transcriptase
DNA molecules have come under the spotlight as potential templates for the fabrication of nanoscale products, such as molecular‐scale electronic or photonic devices. Herein, we report an enhanced approach for the synthesis of oligoblock copolymer‐type DNA by using the Klenow fragment exonuclease minus of E. coli DNA polymerase I (KF − ) in a multi‐step reaction with natural and unnatural nucleotides. First, we confirmed the applicability of unnatural nucleotides with 7‐deaza‐nucleosides—which was expected because they were non‐metalized nucleotides—on the unique polymerization process known as the “strand‐slippage model”. Because the length of the DNA sequence could be controlled by tuning the reaction time, analogous to a living polymerization reaction on this process, stepwise polymerization provided DNA block copolymers with natural and unnatural bases. AFM images showed that this DNA block copolymer could be metalized sequence‐selectively. This approach could expand the utility of DNA as a template.