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A Cresyl Violet‐Based Fluorescent Off–On Probe for the Detection and Imaging of Hypoxia and Nitroreductase in Living Organisms
Author(s) -
Wan QiongQiong,
Gao XingHui,
He XinYuan,
Chen SuMing,
Song YanChao,
Gong QiuYu,
Li XiaoHua,
Ma HuiMin
Publication year - 2014
Publication title -
chemistry – an asian journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.18
H-Index - 106
eISSN - 1861-471X
pISSN - 1861-4728
DOI - 10.1002/asia.201402364
Subject(s) - nitroreductase , cresyl violet , fluorescence , chemistry , biophysics , detection limit , hypoxia (environmental) , zebrafish , biochemistry , oxygen , biology , optics , chromatography , staining , enzyme , genetics , physics , organic chemistry , gene
A new cresyl violet‐based fluorescent off–on probe has been developed through a one‐step synthesis for the detection of nitroreductase (NTR) and hypoxia. The detection mechanism is based on the NTR‐catalyzed reduction of the probe to cresyl violet, accompanied with a large fluorescence enhancement at a long wavelength of 625 nm. The probe can detect NTR in aqueous solution with high selectivity and sensitivity, and the detection limit is 1 ng mL −1 NTR. Most importantly, the probe has been successfully used to image not only NTR and hypoxia in living cells, but also the distribution of NTR in zebrafish in vivo.